Universal miRNA Cloning Linker from New England Biolabs

Product Specs
ItemUniversal miRNA Cloning Linker
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberS1315S
TargetUniversal miRNA Cloning Linker
Quantity5 µg
ApplicationsFunctional Genomics
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Transcriptome-wide analysis of the function of Ded1 in translation preinitiation complex assembly in a reconstituted in vitro systembioRxivOctober 20, 2023Fujun Zhou, Julie M. Bocetti, Meizhen Hou, Daoming Qin, Alan G. Hinnebusch, Jon R. Lorsch50–90 nt fragments for constructing a sequencing library using Universal miRNA Cloning Linker (NEB; S1315S) and the RNA-Seq library construction procedures described above. Sequencing was done on an Illumina

(2) Protocol to measure ribosome density along mRNA transcripts of Arabidopsis thaliana tissues using Ribo-seqSTAR ProtocolsSeptember 1, 2023Isabel Cristina Vélez-Bermúdez, Shu-Jen Chou, Ai-Ping Chen, Wen-Dar Lin, Wolfgang Schmidtbuffer",New England Biolabs,Cat#M0242L Universal miRNA cloning linker (Linker #1),New England Biolabs,Cat#S1315S Novex TBE-urea sample buffer (2×),Thermo Fisher Scientific,Cat#LC6876 Low-range ssRNA ladder,New England Biolabs

(3) Nanopore-based RNA sequencing deciphers the formation, processing, and modification steps of rRNA intermediates in archaeaRNAAugust 1, 2023Felix Grünberger, Michael Jüttner, Robert Knüppel, Sébastien Ferreira-Cerca, Dina Grohmanna custom 3′ cDNA RT primer. Accordingly, a custom 3′ adapter (5′-rAppCTGTAGGCACCATCAAT-NH2-3′, NEB, S1315S) was ligated to all RNAs, following the protocol described in Mo et al. (2021). Briefly, 100 ng pretreated

(4) Accurate transcriptome-wide identification and quantification of alternative polyadenylation from RNA-seq data with APAIQGenome ResearchApril 28, 2023Yongkang Long, Bin Zhang, Shuye Tian, Jia Jia Chan, Juexiao Zhou, Zhongxiao Li, Yisheng Li, Zheng An, Xingyu Liao, Yu Wang, et al.R1015). The rRNA-depleted RNA (5 μL) was then mixed with 1 μL 50 pmol Universal miRNA cloning linker (NEB S1315S; 5′ rAppCTGTAGGCACCATCAAT–NH2 3′) at 65°C for 5 min, and then ligated using T4 RNA Ligase 2, truncated KQ

(5) Codon optimality has minimal effect on determining translation efficiency in mycobacterium tuberculosisScientific ReportsJanuary 9, 2023Smitha Soman, Somdeb Chattopadhyay, Siya Ram, Vinay Kumar Nandicoori, G. Aneeshkumar Arimbasseri(CIP),MO290S,NEB CircLigase II ssDNA ligase,CL4115K,Epicenter biotechnologies Universal miRNA cloning linker,S1315S,NEB Formaldehyde,F8775,Sigma-Aldrich Reagents used in this study. The source and catalog number

(6) TGS1 impacts snRNA 3′-end processing, ameliorates survival motor neuron-dependent neurological phenotypes in vivo and prevents neurodegenerationNucleic Acids ResearchNovember 28, 2022Lu Chen, Caitlin M Roake, Paolo Maccallini, Francesca Bavasso, Roozbeh Dehghannasiri, Pamela Santonicola, Natalia Mendoza-Ferreira, Livia Scatolini, Ludovico Rizzuti, Alessandro Esposito, et al.depleted RNA was ligated to 5 μM of 5′ adenylated, 3′ blocked adaptor (Universal miRNA cloning linker, NEB S1315S) with 250 U of T4 RNA ligase-truncated KQ (NEB M0373S), 25% polyethylene glycol (PEG) 8000, and 1 μl

(7) Global and gene-specific translational regulation in Escherichia coli across different conditionsPLOS Computational BiologyOctober 20, 2022Di Zhang, Sophia Hsin-Jung Li, Christopher G. King, Ned S. Wingreen, Zemer Gitai, Zhiyuan Liend by PNK (M0201, NEB). The repaired fragments were linked to the Universal miRNA Cloning Linker (S1315S, NEB), reverse transcribed (18080044, Thermo), and circularized (CL4111K, Epicentre). rRNA was subtracted

(8) A custom library construction method for super-resolution ribosome profiling in ArabidopsisPlant MethodsOctober 4, 2022Hsin-Yen Larry Wu, Polly Yingshan Hsuvia T4 PNK (NEB M0201S) in the absence of ATP and ligated to a universal miRNA cloning linker (NEB S1315S) with T4 RNA Ligase 2 truncated K227Q (NEB M0351S). The excess linkers were removed with 5′ deadenylase

(9) A stem cell roadmap of ribosome heterogeneity reveals a function for RPL10A in mesoderm productionNature communicationsSeptember 19, 2022Genuth NR, Shi Z, Kunimoto K, Hung V, Xu AF, Kerr CH, Tiu GC, Oses-Prieto JA, Salomon-Shulman REA, Axelrod JD, et al.°C for 20 min. Samples were then incubated with 0.5 μL of 50 μM Universal miRNA Cloning Linker (NEB S1315S), denatured at 65 °C for 5 min, and mixed with 1 μL T4 RNA Ligase 2, truncated KQ (NEB M0373S), 1 μL

(10) Oligodendrocyte differentiation alters tRNA modifications and codon optimality-mediated mRNA decayNature communicationsAugust 25, 2022Martin S, Allan KC, Pinkard O, Sweet T, Tesar PJ, Coller J.mM Tris-HCl pH8. A preadenylylated linker (1 μL of 0.5 μg/μL) (Universal miRNA Cloning Linker, NEB, S1315S) was then ligated after a brief denaturation of 90 s at 80 °C, with T4 truncated RNA ligase 2 (T4 Rnl2(tr),

(11) A transcriptional roadblock protects yeast centromeresNucleic Acids ResearchAugust 12, 2022Sabrine Hedouin, Glennis A Logsdon, Jason G Underwood, Sue Bigginsaccording to the manufacturer's instructions. RNA was ligated to the universal miRNA cloning linker (NEB, #S1315S) by combining 100 ng of poly(A)-enriched RNA with 1 μM universal miRNA cloning linker, 1X T4 RNA ligase

(12) Direct epitranscriptomic regulation of mammalian translation initiation through N4-acetylcytidineMolecular cellAugust 4, 2022Arango D, Sturgill D, Yang R, Kanai T, Bauer P, Roy J, Wang Z, Hosogane M, Schiffers S, Oberdoerffer S.EDTA-free",ThermoFisher Scientific,Cat#:32955 Preadenylated universal miRNA cloning linker,New England Biolabs,Cat#:S1315S ProSignal Pico ECL Reagent,Genesee Scientific,Cat#:20-300B Propidium iodide,Roche,Cat#:11348639001 Protein G Magnetic Beads

(13) Ribosomal leaky scanning through a translated uORF requires eIF4G2Nucleic acids researchJanuary 25, 2022Smirnova VV, Shestakova ED, Nogina DS, Mishchenko PA, Prikazchikova TA, Zatsepin TS, Kulakovskiy IV, Shatsky IN, Terenin IM.M0201S), precipitated with isopropanol, ligated to preadenylated Universal miRNA cloning linker (NEB, S1315S) using truncated T4 RNA ligase 2 (NEB, M0242S). The ligated RNA was precipitated with isopropanol, purified

(14) Nuclear poly(A) binding protein 1 (PABPN1) mediates zygotic genome activation-dependent maternal mRNA clearance during mouse early embryonic developmentNucleic Acids ResearchJanuary 11, 2022Long-Wen Zhao, Ye-Zhang Zhu, Yun-Wen Wu, Shuai-Bo Pi, Li Shen, Heng-Yu FanNo. 74106) and ligated overnight at 25°C with a 2 μM Universal miRNA Cloning Linker (NEB, Cat. No. S1315S) using 200 U T4 RNA ligase 2 truncated KQ (NEB, Cat. No. M0373S) in the presence of 25% PEG 8000 and

(15) Deletion of the N-Terminal Domain of Yeast Eukaryotic Initiation Factor 4B Reprograms Translation and Reduces Growth in UreaFrontiers in Molecular BiosciencesJanuary 3, 2022Xiaozhuo Liu, Houtan Moshiri, Qian He, Ansuman Sahoo, Sarah E. Walkerpre-adenylated universal linker (5′-rAppCTGTAGGCACCATCAAT-NH2-3′) was prepared in house or purchased from NEB (S1315S) and ligated to the 3′ ends of the dephosphorylated footprints using T4 RNA Ligase 2, truncated (MO242L,

(16) Landscape of transcription termination in Arabidopsis revealed by single-molecule nascent RNA sequencingGenome BiologyDecember 1, 2021Weipeng Mo et al.(ZYMO, R1013), the nuclear RNA was mixed with 50 pmol 3′ adapter (5′-rAppCTGTAGGCACCATCAAT–NH2-3′, NEB, S1315S) at 65 °C for 5 min, and then placed on ice for more than 1 min. Then, 2 μl 10× T4 RNA ligase reaction buffer

(17) 40S ribosome profiling reveals distinct roles for Tma20/Tma22 (MCT-1/DENR) and Tma64 (eIF2D) in 40S subunit recyclingNature CommunicationsDecember 1, 2021David J. Young, Sezen Meydan, Nicholas R. Guydoshfragments were dephosphorylated using PNK (NEB; M0201L) and ligated to universal miRNA cloning linker (NEB; S1315S) using truncated T4 RNA ligase 2 (NEB; M0242L). The ligated footprints were size selected on a 10% TBE-Urea

(18) An ancient retroviral RNA element hidden in mammalian genomes and its involvement in co-opted retroviral gene regulationRetrovirologyDecember 1, 2021Koichi Kitao, So Nakagawa, Takayuki Miyazawaisolated using RNAzol RT from 293T cells 24 h after transfection. Universal miRNA Cloning Linker (#S1315S, New England Biolabs) was linked to the 3′-terminus of RNA using T4 RNA Ligase 2, truncated K227Q (#M0351S,

(19) Controlling tissue patterning by translational regulation of signaling transcripts through the core translation factor eIF3cDevelopmental cellNovember 8, 2021Fujii K, Zhulyn O, Byeon GW, Genuth NR, Kerr CH, Walsh EM, Barna M.AP (LifeTech, EF0652) and T4 PNK (NEB, M0201L) for dephosphorylation, ligated with 3’ adaptor (NEB, S1315S, Sequence: 5’ rAppCTGTAGGCACCATCAAT-NH2 3’) by T4 RNA ligase 2 truncated (NEB, M0242S), and then radiolabeled

(20) Multiomics interrogation into HBV (Hepatitis B virus)-host interaction reveals novel coding potential in human genome, and identifies canonical and non-canonical proteins as host restriction factors against HBVCell DiscoveryNovember 2, 2021Shilin Yuan, Guanghong Liao, Menghuan Zhang, Yuanfei Zhu, Weidi Xiao, Kun Wang, Chuanyin Li, Caiwei Jia, Na Sun, Axel Walch, et al.M0201S), T4 RNA ligase 2, truncated (NEB, cat. No. M0242S), Universal miRNA Cloning Linker (NEB, cat. No. S1315S), Superscript III (Invitrogen, cat. No 18080-044), Circ Ligase (Epicenter, cat. No. CL4111K), NEBNext®

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